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Reticulocytes Test

ÇÎÊÈÇÑ ÇáÎáÇíÇ ÇáÔÈßíÉ


ÇÎÊÈÇÑ ÇáÎáÇíÇ ÇáÔÈßíÉ

ÊÍÖíÑ ÇáÕÈÛÉ:

-ÊÐÇÈ ÈæÏÑÉ ÇÒÑÞ ÇááãÇÚ ÈãÞÏÑÇ 1 Ìã Ýì 100 ãáì ãÍáæá ãßæä ãä 80 ãáì ãÍáæá ãáÍì(9 Ìã ãáÍ Ýì ÇááÊÑ) æ 20 ãáì ãÍáæá ÓÊÑÇÊ ÕæÏíæã 3.1 %.Ëã íÑÔÍ ÇáãÍáæá ÈÚÏ ÊÍÖíÑå.
-ÊÕÈÛ ÇáÎáíÉ ÇáÔÈßíÉ ÈÇáÇÒÑÞ ÇááãÇÚ ÍÊì íãßä ÊãííÒåÇ Úä ÈÇÞì ßÑÇÊ ÇáÏã ÇáÍãÑÇÁ.


ÇáØÑíÞÉ:

-äÖÚ Ýì ÇäÈæÈÉ æÇÒÑãÇä 3 äÞØ ãä ÕÈÛÉ ÇáÇÒÑÞ ÇááãÇÚ.
-íÖÇÝ ÖÚÝåÇ Ïã Çì 6 äÞØ Ïã ãÃÎæÐÉ Úáì ãÇäÚ ÊÌáØ Ýì ÇáÍÇáÇÊ ÇáÚÇÏíÉ ÃãÇ Ýì ÍÇáÇÊ ÇáÇäíãíÇÁ äÖíÝ 1...Read more
Blood reticulocytes

Gram stain procedure

ØÑíÞÉ ÕÈÛÉ ÌÑÇã (ÕÈÛÉ ÛÑÇã)

This stain for differentiate between G +ve bacteria (which accept staining) and G -ve bacteria (which not accept staining).

Steps:
-Prepare your clear slide and put one drop of normal saline.
-Take specimen from the colony and mix it well with normal saline.
-Leave for dryness on air then fix it on burner.
-Then pour Crystal Violet stain on the slide and leave it for 1 minute.
-Wash gently with water.
-Then pour Iodine stain on the slide and leave it for 1 minute.
-Wash gently w...Read more
Gram stain procedure

Blood group test

ÇáßÔÝ Úä ÝÕíáÉ ÇáÏã

Sample is fresh blood or EDTA blood(anticoagulant)

Put 10 µ of anti A on one side of a slide and put 10 µ of anti B on the other side

Put 10 µ of blood tested in each side and mix the blood with the reagent added.

results:

+ A & + B = AB

+ A & - B = A

- A & + B = B

-A & - B = O ...Read more
Blood Groups