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Antibiotic Susceptibility Test

ÒÑÇÚÉ ÇÎÊÈÇÑ ÞæÉ ÇáãÖÇÏÇÊ ÇáÍíæÉ

This test for testing number of antibiotics against certain organism to find the best one and the most effective on this organism.

Steps:
-Take a swap from the colony by a sterile wire loop and culture it on Muller-hinton agar.
-Leave it incubated for 24 hours at 37ºC.

Result:
You notice now the larger clear zone around an antibiotic means that this antibiotic is the best.
See Samples below......Read more

Tenia

ÇáÊíäíÇ Ãæ ÇáÏæÏÉ ÇáÔÑíØíÉ ÈÇáÇãÚÇÁ



ÇáÏæÏÉ ÇáÔÑíØíÉ:

ÏæÏÉ ØæíáÉ ÞÏ íÕá ØæáåÇ Åáì ÓÊ ÃãÊÇÑ ÊÚíÔ ÈÃãÚÇÁ ÇáÅäÓÇä æÇáÍíæÇä æÊãÊÕ ÇáÛÐÇÁ ÇáãåÖæã æåí ËäÇÆíÉ ÇáÍÇÖä ÍíË íÎÑÌ ÈíÖåÇ ãÚ ÈÑÇÒ ÇáÅäÓÇä Ýí ÇáÚÑÇÁ ( ÓÇÈÞÇð ) ÝíáæË ÇáÃÚÔÇÈ ÇáÊí ÊÃßáåÇ ÇáÃÈÞÇÑ ÝÊÝÞÓ ÇáÈíÖÉ Ýí ÃãÚÇÁ ÇáÃÈÞÇÑ æÊÊÍæá Åáì ÏæÏÉ ÕÛíÑÉ ÊÓÊÞÑ Ýí ÚÖáÇÊ ÇáÈÞÑ.
æÚäÏãÇ ÊÐÈÍ ÇáÃÈÞÇÑ æíÃßá ÇáÅäÓÇä áÍãåÇ ( ÛíÑ ÇáãØåí ÌíÏÇð ) ÊÓÊÞÑ Ýí ÃãÚÇÁ ÇáÅäÓÇä æÊßÈÑ æÊØæá. Åä ÇáÏæÏÉ ÇáÔÑíØíÉ ÈÚÏ Åä ÊãÊÕ ÛÐÇÁ ÇáÅäÓÇä ÊÝÑÒ áå ÝíÊÇãíä È 12 ÝíÈÏæ æÌå Çá...Read more

Gram stain procedure

ØÑíÞÉ ÕÈÛÉ ÌÑÇã (ÕÈÛÉ ÛÑÇã)

This stain for differentiate between G +ve bacteria (which accept staining) and G -ve bacteria (which not accept staining).

Steps:
-Prepare your clear slide and put one drop of normal saline.
-Take specimen from the colony and mix it well with normal saline.
-Leave for dryness on air then fix it on burner.
-Then pour Crystal Violet stain on the slide and leave it for 1 minute.
-Wash gently with water.
-Then pour Iodine stain on the slide and leave it for 1 minute.
-Wash gently w...Read more